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vector pcat3basic  (Promega)

 
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    Promega vector pcat3basic
    Construction of expression-cassette clones containing three single–nucleotide polymorphisms in the APOε promoter sequence. A: Schematic diagram of a 1.4 kb PCR fragment of the APOε gene (small arrows indicate locations of PCR primers), including the 5′-UTR and 1 kb of promoter, and a fragment of the first intron of the gene, as produced by PCR from human genomic DNA samples. Three single-nucleotide polymorphic sites [Bullido et al., 1998; Artiga et al., 1998b; Lambert et al., 2002] are indicated (−491, −427, and −219) in relationship to the transcription start site (+1). An active NF-κB site [Du et al., 2005] and promoter activity domains [Maloney et al., 2007] (HuA-“human A”-through HuE) are also indicated. B: The 1.4 kb fragment was cloned into the KpnI and XhoI sites of pBluescript SK (−). C: The pBluescript-backbone clone was digested with KpnI and XhoI, and the 1.4 kb APOε fragment was subcloned into the KpnI/XhoI sites of pGL3Basic. D: The pGL3Basic-backbone clone was digested with KpnI and XhoI, and the 1.4 kb APOε fragment was cloned into the KpnI/XhoI sites of <t>pCAT3Basic.</t> E: Eight different haplotype APOε promoter-CAT expression gene clones were generated and subsequently used for DNA transfection studies.
    Vector Pcat3basic, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vector pcat3basic/product/Promega
    Average 90 stars, based on 1 article reviews
    vector pcat3basic - by Bioz Stars, 2026-04
    90/100 stars

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    1) Product Images from "Functional Characterization of Three Single-Nucleotide Polymorphisms Present in the Human APOε Promoter Sequence: Differential Effects in Neuronal Cells and on DNA–Protein Interactions"

    Article Title: Functional Characterization of Three Single-Nucleotide Polymorphisms Present in the Human APOε Promoter Sequence: Differential Effects in Neuronal Cells and on DNA–Protein Interactions

    Journal: American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics

    doi: 10.1002/ajmg.b.30973

    Construction of expression-cassette clones containing three single–nucleotide polymorphisms in the APOε promoter sequence. A: Schematic diagram of a 1.4 kb PCR fragment of the APOε gene (small arrows indicate locations of PCR primers), including the 5′-UTR and 1 kb of promoter, and a fragment of the first intron of the gene, as produced by PCR from human genomic DNA samples. Three single-nucleotide polymorphic sites [Bullido et al., 1998; Artiga et al., 1998b; Lambert et al., 2002] are indicated (−491, −427, and −219) in relationship to the transcription start site (+1). An active NF-κB site [Du et al., 2005] and promoter activity domains [Maloney et al., 2007] (HuA-“human A”-through HuE) are also indicated. B: The 1.4 kb fragment was cloned into the KpnI and XhoI sites of pBluescript SK (−). C: The pBluescript-backbone clone was digested with KpnI and XhoI, and the 1.4 kb APOε fragment was subcloned into the KpnI/XhoI sites of pGL3Basic. D: The pGL3Basic-backbone clone was digested with KpnI and XhoI, and the 1.4 kb APOε fragment was cloned into the KpnI/XhoI sites of pCAT3Basic. E: Eight different haplotype APOε promoter-CAT expression gene clones were generated and subsequently used for DNA transfection studies.
    Figure Legend Snippet: Construction of expression-cassette clones containing three single–nucleotide polymorphisms in the APOε promoter sequence. A: Schematic diagram of a 1.4 kb PCR fragment of the APOε gene (small arrows indicate locations of PCR primers), including the 5′-UTR and 1 kb of promoter, and a fragment of the first intron of the gene, as produced by PCR from human genomic DNA samples. Three single-nucleotide polymorphic sites [Bullido et al., 1998; Artiga et al., 1998b; Lambert et al., 2002] are indicated (−491, −427, and −219) in relationship to the transcription start site (+1). An active NF-κB site [Du et al., 2005] and promoter activity domains [Maloney et al., 2007] (HuA-“human A”-through HuE) are also indicated. B: The 1.4 kb fragment was cloned into the KpnI and XhoI sites of pBluescript SK (−). C: The pBluescript-backbone clone was digested with KpnI and XhoI, and the 1.4 kb APOε fragment was subcloned into the KpnI/XhoI sites of pGL3Basic. D: The pGL3Basic-backbone clone was digested with KpnI and XhoI, and the 1.4 kb APOε fragment was cloned into the KpnI/XhoI sites of pCAT3Basic. E: Eight different haplotype APOε promoter-CAT expression gene clones were generated and subsequently used for DNA transfection studies.

    Techniques Used: Expressing, Clone Assay, Sequencing, Produced, Activity Assay, Generated, Transfection



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    vector pcat3basic  (Promega)
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    Promega vector pcat3basic
    Construction of expression-cassette clones containing three single–nucleotide polymorphisms in the APOε promoter sequence. A: Schematic diagram of a 1.4 kb PCR fragment of the APOε gene (small arrows indicate locations of PCR primers), including the 5′-UTR and 1 kb of promoter, and a fragment of the first intron of the gene, as produced by PCR from human genomic DNA samples. Three single-nucleotide polymorphic sites [Bullido et al., 1998; Artiga et al., 1998b; Lambert et al., 2002] are indicated (−491, −427, and −219) in relationship to the transcription start site (+1). An active NF-κB site [Du et al., 2005] and promoter activity domains [Maloney et al., 2007] (HuA-“human A”-through HuE) are also indicated. B: The 1.4 kb fragment was cloned into the KpnI and XhoI sites of pBluescript SK (−). C: The pBluescript-backbone clone was digested with KpnI and XhoI, and the 1.4 kb APOε fragment was subcloned into the KpnI/XhoI sites of pGL3Basic. D: The pGL3Basic-backbone clone was digested with KpnI and XhoI, and the 1.4 kb APOε fragment was cloned into the KpnI/XhoI sites of <t>pCAT3Basic.</t> E: Eight different haplotype APOε promoter-CAT expression gene clones were generated and subsequently used for DNA transfection studies.
    Vector Pcat3basic, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vector pcat3basic/product/Promega
    Average 90 stars, based on 1 article reviews
    vector pcat3basic - by Bioz Stars, 2026-04
    90/100 stars
    		  Buy from Supplier
    pcat3basic vector  (Promega)
    90
    Promega pcat3basic vector
    Construction of expression-cassette clones containing three single–nucleotide polymorphisms in the APOε promoter sequence. A: Schematic diagram of a 1.4 kb PCR fragment of the APOε gene (small arrows indicate locations of PCR primers), including the 5′-UTR and 1 kb of promoter, and a fragment of the first intron of the gene, as produced by PCR from human genomic DNA samples. Three single-nucleotide polymorphic sites [Bullido et al., 1998; Artiga et al., 1998b; Lambert et al., 2002] are indicated (−491, −427, and −219) in relationship to the transcription start site (+1). An active NF-κB site [Du et al., 2005] and promoter activity domains [Maloney et al., 2007] (HuA-“human A”-through HuE) are also indicated. B: The 1.4 kb fragment was cloned into the KpnI and XhoI sites of pBluescript SK (−). C: The pBluescript-backbone clone was digested with KpnI and XhoI, and the 1.4 kb APOε fragment was subcloned into the KpnI/XhoI sites of pGL3Basic. D: The pGL3Basic-backbone clone was digested with KpnI and XhoI, and the 1.4 kb APOε fragment was cloned into the KpnI/XhoI sites of <t>pCAT3Basic.</t> E: Eight different haplotype APOε promoter-CAT expression gene clones were generated and subsequently used for DNA transfection studies.
    Pcat3basic Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcat3basic vector/product/Promega
    Average 90 stars, based on 1 article reviews
    pcat3basic vector - by Bioz Stars, 2026-04
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Construction of expression-cassette clones containing three single–nucleotide polymorphisms in the APOε promoter sequence. A: Schematic diagram of a 1.4 kb PCR fragment of the APOε gene (small arrows indicate locations of PCR primers), including the 5′-UTR and 1 kb of promoter, and a fragment of the first intron of the gene, as produced by PCR from human genomic DNA samples. Three single-nucleotide polymorphic sites [Bullido et al., 1998; Artiga et al., 1998b; Lambert et al., 2002] are indicated (−491, −427, and −219) in relationship to the transcription start site (+1). An active NF-κB site [Du et al., 2005] and promoter activity domains [Maloney et al., 2007] (HuA-“human A”-through HuE) are also indicated. B: The 1.4 kb fragment was cloned into the KpnI and XhoI sites of pBluescript SK (−). C: The pBluescript-backbone clone was digested with KpnI and XhoI, and the 1.4 kb APOε fragment was subcloned into the KpnI/XhoI sites of pGL3Basic. D: The pGL3Basic-backbone clone was digested with KpnI and XhoI, and the 1.4 kb APOε fragment was cloned into the KpnI/XhoI sites of pCAT3Basic. E: Eight different haplotype APOε promoter-CAT expression gene clones were generated and subsequently used for DNA transfection studies.

    Journal: American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics

    Article Title: Functional Characterization of Three Single-Nucleotide Polymorphisms Present in the Human APOε Promoter Sequence: Differential Effects in Neuronal Cells and on DNA–Protein Interactions

    doi: 10.1002/ajmg.b.30973

    Figure Lengend Snippet: Construction of expression-cassette clones containing three single–nucleotide polymorphisms in the APOε promoter sequence. A: Schematic diagram of a 1.4 kb PCR fragment of the APOε gene (small arrows indicate locations of PCR primers), including the 5′-UTR and 1 kb of promoter, and a fragment of the first intron of the gene, as produced by PCR from human genomic DNA samples. Three single-nucleotide polymorphic sites [Bullido et al., 1998; Artiga et al., 1998b; Lambert et al., 2002] are indicated (−491, −427, and −219) in relationship to the transcription start site (+1). An active NF-κB site [Du et al., 2005] and promoter activity domains [Maloney et al., 2007] (HuA-“human A”-through HuE) are also indicated. B: The 1.4 kb fragment was cloned into the KpnI and XhoI sites of pBluescript SK (−). C: The pBluescript-backbone clone was digested with KpnI and XhoI, and the 1.4 kb APOε fragment was subcloned into the KpnI/XhoI sites of pGL3Basic. D: The pGL3Basic-backbone clone was digested with KpnI and XhoI, and the 1.4 kb APOε fragment was cloned into the KpnI/XhoI sites of pCAT3Basic. E: Eight different haplotype APOε promoter-CAT expression gene clones were generated and subsequently used for DNA transfection studies.

    Article Snippet: The 1.4 kb APOE promoter fragment band was purified from each and subcloned into the Kpn I and Xho I sites of vector pCAT3Basic (Promega) to produce eight fusion clones, containing each possible permutation of the SNP variants, driving the CAT reporter coding sequence ( ) to produce eight different polymorphic haplotype clones ( ). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG. 1 caption a7 Construction of expression-cassette clones containing three single–nucleotide polymorphisms in the APOε promoter sequence.

    Techniques: Expressing, Clone Assay, Sequencing, Produced, Activity Assay, Generated, Transfection